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Progress in Stem Cell

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Expression of CD34 marker in the adipose tissue derived stem cells

Phuc Van Pham 1, 2, *
Ngoc Bich Vu 2
Van Hong Tran 2

  1. Laboratory of Stem Cell Research and Application, VNUHCM University of Science, Viet Nam
  2. Stem Cell Institute, VNUHCM University of Science, Viet Nam
Correspondence to: Phuc Van Pham, Laboratory of Stem Cell Research and Application, VNUHCM University of Science, Viet Nam; Stem Cell Institute, VNUHCM University of Science, Viet Nam. Email: pvphuc@hcmuns.edu.vn.
Volume & Issue: Vol. 5 No. 1 (2018) | DOI: 10.15419/psc.v5i1.399
Published: 2018-03-01

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Copyright © 2018 by the author(s).

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Introduction: Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marker of hematopoietic stem cells. This study aimed to analyze and determine the expression of CD34 marker in ten samples of ADSCs obtained from 10 donors.

Methods: All ADSC samples were isolated and expanded according to the published previous protocols. They were confirmed as the MSCs with some markers and differentiation potential, excepting the CD34 expression. Then they were cultured and analyzed the expression of CD34 by flow cytometry at passage 3, 5, 7 and 9.

Results: The results showed that expression of CD34 in ADSCs was different between donors and their passages that accounted from 1.21% to 23.38%.

Conclusion: These results suggested that ADSCs are not ‘truly” MSCs like MSCs from bone marrow.

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