Progress in Stem Cell
type: channel
description: Progress in Stem Cell (PSC) (ISSN 2199-4633) is the best Open Access journal that acts as a forum for translational research into stem cell therapies. PSC is scientific journal that overlays the study of Cancer stem cells, Stem cell therapy, Stem-cell transplantation, human embryonic stem cells, Neural stem cells, Murine Embryonic Stem Cells, Adult stem cell, Pancreatic stem cells, etc. PSC is a peer-reviewed journal that focuses on the areas of established and emerging concepts in stem cell research and their assorted disease therapies. It provides an opportunity to share the scientific information among the clinical & medical scientists and researchers.
publisher: Biomedpress
language: en
publication name: Progress in Stem Cell
issn: 2199-4633
copyright: Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
items: The Effect of the Dental Stem Cell Secretome on Tissue Regeneration: A Systematic Review
items: L-ascorbic acid-2-phosphate and Fibrin Substrate Enhances the Chondrogenic Differentiation of Human Adipose-derived Stem Cells
items: Present Status of Research on the Regenerative Potential of Dental Pulp Stem Cells in Malaysia: A Systematic Review
items: Good manufacturing practice-compliant isolation and culture of human bone marrow mesenchymal stem cells
items: What are markers for breast cancer stem cells ?
items: Mesenchymal Stem Cells: vector for targeted cancer therapy
items: In vitro expansion of mesenchymal stem cells for clinical use
items: STEM CELL AND CANCER STEM CELL: A Tale of Two Cells
items: Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering- a Perspective
items: The progress of Stem cells in the treatment of diabetes mellitus type 1
items: Co-injection of ETV-2 and HGF viral vectors significantly improve angiogenesis in mice with hindlimb ischemia
items: Transplantation of umbilical cord blood-derived mesenchymal stem cells to treat liver cirrhosis in mice: a comparison of tail and portal vein injection
items: Concise review: Hematopoietic stem cell transplantation to treat insulin-dependent diabetes mellitus
items: Conversion of human adipose derived stem cells into endothelial progenitor cells
items: Concise review: Umbilical cord derived mesenchymal stem cell bank
items: Expanded mesenchymal stem cell transplantation is safe in both local injection and vein transfusion
items: An evolution of stem cell research and therapy in Viet Nam
items: Expression of CD34 marker in the adipose tissue derived stem cells
items: Mesenchymal stem cells transplantation reduces diabetic nephropathy
items: Therapeutic role of bone marrow-derived stem cells and zinc sulfate for reduction of liver fibrosis
items: https://cellstemcell.org/index.php/PSC/article/view/407
items: Ovarian cancer cells with CD133+ phenotype is more resistant against Ngai Bun Boesenbergia pandurata extract than original ovarian cancer cells
items: Induced pluripotent stem cells are Japanese brand sources for therapeutic cells to pretrial clinical research
items: Using Defined Neural Cell Populations as a Possible Solution for Challenges in Neural Stem Cell Therapy
items: Recent Progress in Stem Cell Chondrogenesis
items: Demons resurrected from Angels! What are or are not the molecular clues of the rising of the teratoma or teratocarcinoma from human amniotic stem cell?
items: Adipose tissue derived stromal vascular fraction transplantation can recover spinal cord injury in mice
items: Autologous and allogeneic transplantation of adipose derived stem cells have similar efficacy for type 1 diabetes mellitus therapy in mouse models
items: Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector
items: Dissecting asthma pathogenesis through study of patterns of cellular traffic indicative of molecular switches operative in inflammation

The Effect of the Dental Stem Cell Secretome on Tissue Regeneration: A Systematic Review
type: item
description: Secretome therapy is a promising approach in tissue regeneration because it can reproduce most of the advantages of cell-based therapies. This review aims to investigate the most prominent effect of using dental-derived secretome on tissue regeneration using a systematic review approach. A systematic electronic search was conducted via the PubMed, Scopus and Wiley online library databases for studies published in English up to October 2020. All the articles from the databases were screened, and the criteria for inclusion and exclusion were applied. Forty papers were included in the study, whereby there were 16 in vitro studies and 11 in vivo studies with different animal models. No clinical trial has been reported yet. The most studied DSCs were human SHEDs (12 studies), followed by human DPSCs (11) and human PDLSCs (5). The majority of the studies used secretome from human SHEDs and DPSCs. TGF-ß 1 is the most frequently detected protein in the secretome, which comes from six types of DSCs, followed by NGF and NT-3, which were found in five different types of DSC secretome. The compositions of the secretome were found to promote the regeneration of the tissues through their neurogenic, angiogenic, osteogenic and odontogenic effects, with the majority of studies reviewed reporting using them for nondental tissue regeneration. From this review, DSC-CM reported favorable tissue regeneration potential; however, many factors need to be explored in future research with regard to the application of secretome delivery, particularly DSC-CM, in the clinical setting.
subject: Reviews
  topic: section
creator: Nusaibah Sakinah Nordin
creator: Maryati Md Dasor
creator: Farha Ariffin
creator: Zurairah Berahim
creator: Muhammad Huzaimi Haron
rights: Copyright (c) 2022 Nusaibah Sakinah Nordin, Maryati Md Dasor, Farha Ariffin, Zurairah Berahim, Muhammad Huzaimi Haron
date: 2022-11-14
publication date: 2022-11-14
starting page: 318
ending page: 336
doi: 10.15419/psc.v9i1-2.413

L-ascorbic acid-2-phosphate and Fibrin Substrate Enhances the Chondrogenic Differentiation of Human Adipose-derived Stem Cells
type: item
description: Background: Adipose-derived stem cells (ASCs) have been developed as a substitute for chondrocytes in cartilage tissue engineering. This study aimed to evaluate the potency of ascorbic acid to induce the chondrogenic differentiation of human ASCs cultured on fibrin substrate. Methods: Human ASCs were isolated by an enzymatic process and characterized. The fibrin scaffold was fabricated from human blood as a natural scaffold. The ASCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 mg/ml) and a control without an ascorbic acid supplementation. The chondrogenic differentiation of the ASCs was evaluated involving glycosaminoglycan analysis, and the mRNA expression of type 2 collagen, aggrecan and type 1 collagen. Results: Chondrogenic differentiation occurred when the ASCs were supplemented by 3 mg/ml of ascorbic acid and cultured on a fibrin scaffold confirmed by glycosaminoglycan (GAG) analysis, and the mRNA expression of type 2 collagen and aggrecan. The expression of type 1 collagen, which is an osteogenic marker, was low. Conclusions: ASCs with a supplementation of 3mg/ml of ascorbic acid cultured on a fibrin substrate promotes chondrogenic differentiation.
subject: Research Articles
  topic: section
subject: ASCs
  topic: keywords
subject: L-ascorbic acid-2-phosphate
  topic: keywords
subject: chondrogenesis
  topic: keywords
subject: fibrin
  topic: keywords
subject: differentiation
  topic: keywords
subject: stem cell
  topic: subjects
subject: regenerative medicine
  topic: subjects
creator: Imam Rosadi
creator: Karina Karina
creator: Viol Dhea Kharisma
creator: Arif Nur Muhammad Ansori
rights: Copyright (c) 2022 Imam Rosadi, Karina Karina, Viol Dhea Kharisma, Arif Nur Muhammad Ansori
date: 2021-12-31
publication date: 2021-12-31
starting page: 310
ending page: 317
doi: 10.15419/psc.v8i2.412

Present Status of Research on the Regenerative Potential of Dental Pulp Stem Cells in Malaysia: A Systematic Review
type: item
description: Stem cells from human exfoliated deciduous teeth (SHED) or dental pulp stem cells (DPSCs) from permanent teeth are considered promising sources of mesenchymal stem cells. It requires a less invasive technique to isolate stem cells from exfoliated or permanent teeth. Hence this study aimed to identify the present status of research on the regenerative potential of SHED/DPSCs in Malaysia. The results indicate that only 60 articles were published in regenerative medicine from Malaysia till 5th July 2019. Only 16 tertiary institutes and four industries/clinics were involved in these studies. A poor pattern of collaboration has also been identified. Outcomes of this study have emphasized the conduction of more research on the regenerative potential of SHED/DPSCs, and active collaboration among the tertiary institutes and industries for successful translation of these cells from bench side to bedside.
subject: Reviews
  topic: section
subject: awareness
  topic: keywords
subject: collaboration
  topic: keywords
subject: differentiation
  topic: keywords
subject: DPSC
  topic: keywords
subject: regenerative medicine
  topic: keywords
subject: SHED
  topic: keywords
creator: Wong Mun Xuan
creator: Khoo Chiann
creator: Md. Sanower Hossain
creator: Muhamad Fareez Ismail
creator: Nazmul Haque
rights: Copyright (c) 2021 Nazmul Haque
date: 2021-04-30
publication date: 2021-04-30
starting page: 304
ending page: 309
doi: 10.15419/psc.v8i1.410

Good manufacturing practice-compliant isolation and culture of human bone marrow mesenchymal stem cells
type: item
description: Bone marrow mesenchymal stem cells (BM-MSCs) are multipotent stem cells that can differentiate into some kinds of mesenchymal cells such as osteoblasts, chondroblasts and adipocytes. These cells were discovered for a long time and considered as the first discovered source of MSCs. BM-MSC transplantation was used to treat some diseases such as bone disease, myocardial infarction, stroke, diabetes mellitus… This study aimed to provide a new method to in vitro primarily culture and secondary culture of BM-MSCs that were compliant with good manufacturing practice for clinical applications. Bone marrow was aspirated from the hip bone using special needles and syringes. Mononuclear cells (MNCs) were isolated from bone marrow by BM-MSC extraction kit. These MNCs were cultured in the commercial medium – MSC-Cult supplemented with 10% activated platelet rich plasma (aPRP) to obtain BM-MSCs. The results showed that 100% samples of bone marrow can be successfully cultured to produce BM-MSCs. Obtained cells exhibited the MSC phenotypes, and maintained the stable and normal karyotype up to 10th passages, and failed to cause tumor in the mouse models. This research provided an advanced protocol for clinical applications of BM-MSCs.
subject: Research Articles
  topic: section
subject: Bone marrow mesenchymal stem cells
  topic: keywords
subject: Clinical application
  topic: keywords
subject: GMP
  topic: keywords
subject: Mesenchymal stem cells
  topic: keywords
subject: Stem cell culture
  topic: keywords
subject: Stem cell
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Phuc Van Pham
creator: Nhan Lu-Chinh Phan
creator: Dung Minh Le
creator: Phuong Thi-Bich Le
creator: Tung Dang-Xuan Tran
creator: Ngoc Kim Phan
rights: Copyright (c) 2014 Phuc Van Pham, Nhan Lu-Chinh Phan, Dung Minh Le, Phuong Thi-Bich Le, Tung Dang-Xuan Tran, Ngoc Kim Phan
date: 2014-12-24
publication date: 2014-12-24
starting page: 18
ending page: 27
doi: 10.15419/psc.v1i01.117

What are markers for breast cancer stem cells ?
type: item
description: Breast cancer stem cells were firstly discovered by Al-Hajj et al. (2003). Many scientists interested in targeting them as an important solution to "remove" the root of breast cancer. However, more and more publications showed that breast cancer stem cells are the heterogenous population that expression of some markers can be different between them. Particularly, some different markers were considered as markers of breast cancer stem cells. Therefore, what are gold standards of these cells? In this publication, we discussed some conflicts about markers of breast cancer stem cells and suggested some gold standards for breast cancer stem cells.
subject: Reviews
  topic: section
subject: stem cell
  topic: keywords
subject: cancer
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Phuc Van Pham
creator: Nhan Lu-Chinh Phan
rights: Copyright (c) 2016 Phuc Van Pham, Nhan Lu-Chinh Phan
date: 2016-03-20
publication date: 2016-03-20
starting page: 65
ending page: 72
doi: 10.15419/psc.v3i01.121

Mesenchymal Stem Cells: vector for targeted cancer therapy
type: item
description: Mesenchymal stem cells (MSCs) have been studied extensively due to their potential to differentiate to cell types of varying lineages.Adipose tissue and umbilical cord blood are two tissues frequently used to obtain MSCs. Due to tumor tropism of MSCs and their ability to protect encoded cytotoxic genes, MSCs have garnered interest as a potential vector for targeted therapy, with limited damage to normal tissues. The tumor microenvironment plays a critical role in ensuring the survival of cancer cells through promotion of MSCs to differentiate into cancer-associated fibroblasts (CAFs), which promote tumor growth and metastasis. Through specific interactions between ligands and receptors expressed on MSCs and cancer cells, respectively,MSCs can home to necrotic tissues or inflamed sites in the body, including the tumor microenvironment. In fact, an inflammatory tumor environment is similar to a wound healing environment. This review discusses the preeminent characteristics of MSCs and their influence ontumor cell growth and metastasis. MSCs may represent an encouraging platform for cancer treatment. The combination of MSC and gene therapy represents a potentially outstanding strategy to specifically target and effectively destroy tumor.
subject: Reviews
  topic: section
subject: stem cell
  topic: keywords
subject: cancer
  topic: keywords
creator: Binh Thanh Vu
creator: Ngoc Kim Phan
creator: Phuc Van Pham
rights: Copyright (c) 2016 Binh Thanh Vu, Ngoc Kim Phan, Phuc Van Pham
date: 2016-03-25
publication date: 2016-03-25
starting page: 73
ending page: 86
doi: 10.15419/psc.v3i01.122

In vitro expansion of mesenchymal stem cells for clinical use
type: item
description: Mesenchymal stem cells (MSCs) now are popular stem cells for clinical applications. To date, MSCs were accepted in various disease treatments with several FDA approved treatments in some countries. One important requirement for the clinical usage of stem cells is the production of stem cells. Actually, the treatment efficacy of MSC transplantation depends on the quality of transplanted MSCs. This review aimed to present some guidelines for MSC production according to good manufacturing practice that helps to maintain the quality of stem cells from batch to batch as well as the clinical satisfaction.
subject: Reviews
  topic: section
subject: stem cell
  topic: keywords
subject: regenerative medicine
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Phuc Van Pham
creator: Ngoc Bich Vu
rights: Copyright (c) 2016 Phuc Van Pham, Ngoc Bich Vu
date: 2016-06-24
publication date: 2016-06-24
starting page: 87
ending page: 96
doi: 10.15419/psc.v3i02.123

STEM CELL AND CANCER STEM CELL: A Tale of Two Cells
type: item
description: Stem cells are vital for regenerating and repairing the organs such as the heart, lungs, skin, germ cells and other tissues. But the characteristics of normal stem cells and the discovery of origin of leukemia have headed the scientist to the hypothesis that cancer may initiate from stem cell-like cells. Cancer stem cells are now thought to be responsible for cancer initiation, progression, metastasis, recurrence and drug resistance. There are some similarities and differences between CSCs and healthy stem cells in signaling pathways (Wnt/ β–catenin, Hh, Notch), epigenetic modifications, telomere maintenance, and degree of dependence on the stem cell niche, maintenance of multipotency and self-renewability of respective progenitor cells. Distinct and specific surface biomarker phenotypes can be used to distinguish CSC from other tumor cells and normal stem cells through single and combination of markers such as CD44, CD34+CD38− and CD133. Recently, miRNAs have been tried as a marker and therapeutic agents against cancers stem cell such as miR-34a, miR-199a, miR-181, miR-125b-2 and miR-128. However, targeting CSCs without harming their normal counterparts is an advantageous way in the design of novel therapies but many questions remain unknown.
subject: Reviews
  topic: section
subject: biology
  topic: keywords
subject: stem cell
  topic: keywords
subject: cancer
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: MdShaifur Rahman
creator: Hossen Mohammad Jamil
creator: Naznin Akhtar
creator: K.M.T. Rahman
creator: Rashedul Islam
creator: S.M. Asaduzzaman
rights: Copyright (c) 2016 MdShaifur Rahman, Hossen Mohammad Jamil, Naznin Akhtar, K.M.T. Rahman, Rashedul Islam, S.M. Asaduzzaman
date: 2016-06-24
publication date: 2016-06-24
starting page: 97
ending page: 108
doi: 10.15419/psc.v3i02.124

Culture Methods and Choice of Cells Makes a Difference in Outcome of Tissue Engineering- a Perspective
type: item
description: This perspective reviews the effects of different tissue engineering techniques, for induced differentiation of human embryonic stem cells into pulmonary lineage specific cells, and validating cell based regenerative therapy to ameliorate pulmonary degeneration. The etiology of degeneration differs from disease to disease. Likewise, strategies to regenerate lost tissues in its heterogeneity, should also cater to the regeneration process. To customize the same, researchers ought to device strategies to repair, replace or regenerate, in the spatio- temporal format, healthy tissues in their heterogeneous multi-functional existence, such that when transplanted in vitro, they may find their niche and integrate seamlessly into the tissue system and manifest full-fledged functional competence in order to reverse the erstwhile effects of degeneration. Cell based lung repair or regeneration is unquestionably the most promising agenda of regenerative medicine. In one of our studies, human embryonic stem cell (hESC) line H7 was successfully differentiated into three non-ciliated alveolar epithelial cells. In another study, BJNhem19 and BJNhem20 stem cell lines were used to induce similar guided endodermal differentiation into pulmonary lineage cells. Both studies were done using similar protocols, where the hESCs were first plated on gamma irradiated mouse embryonic fibroblast (MEF) feeder cells and undifferentiated hESCs were taken through embroid body formation. They were then subjected to induction into lung specific cells, by defined growth factors in Small Airways Growth Medium (SAGM) and Bronchiolar Endothelial Growth Media (BEGM). The H7 cells were found to express, both intracellularly and on their surfaces, characteristic marker proteins. However, the BJNhem19 and BJNhem20 cells showed poor differential potential, and could not be successfully differentiated into lung- specific cells. This difference in differentiation potential may be due to several intrinsic limitat…
subject: Reviews
  topic: section
subject: Stem cells
  topic: keywords
subject: bone marrow derived stem cells
  topic: keywords
subject: embryonic stem cells
  topic: keywords
subject: undifferentiated cells
  topic: keywords
subject: lineage specific differentiation
  topic: keywords
subject: embroid body
  topic: keywords
subject: colony forming units
  topic: keywords
subject: induction medium
  topic: keywords
subject: stem cell
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Srabani Kar
creator: Shinjini Mitra
creator: Ena Ray Banerjee
rights: Copyright (c) 2016 Srabani Kar, Shinjini Mitra, Ena Ray Banerjee
date: 2016-09-15
publication date: 2016-09-15
starting page: 109
ending page: 128
doi: 10.15419/psc.v3i03.127

The progress of Stem cells in the treatment of diabetes mellitus type 1
type: item
description: Diabetes mellitus is a major health problem in the world. The total number of diabetic’s population is increasing every year. Currently used treatment of diabetes mellitus type 1 by controlling the blood sugar levels, doesn’t prevent complications which associate diabetes. The stem cell based therapy for diabetes aims to replace the diseased or lost cells of the pancreas with new cells using pluripotent or multipotent stem cells. Scientists successfully produced insulin secreting cell from different types of stem cells. In this article we briefly reviewed the progress made in the stem cell research for diabetes treatment.
subject: Reviews
  topic: section
subject: Diabetes mellitus
  topic: keywords
subject: Cell therapy
  topic: keywords
subject: Stem cell
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Nihad Elsadig Babiker
creator: Alsadig Gassoum
creator: Nahla E. Abdelraheem
creator: Mohamed Abdelrahman Arbab
creator: Sawsan Ahmed Hamed ALDeaf
creator: Mohamad Ahmed Ali El-Sheikh
creator: Hassan Hussein Musa
rights: Copyright (c) 2017 Nihad Elsadig Babiker, Alsadig Gassoum, Nahla E. Abdelraheem, Mohamed Abdelrahman Arbab, Sawsan Ahmed Hamed ALDeaf, Mohamad Ahmed Ali El-Sheikh, Hassan Hussein Musa
date: 2017-06-30
publication date: 2017-06-30
starting page: 175
ending page: 188
doi: 10.15419/psc.v4i01.184

Co-injection of ETV-2 and HGF viral vectors significantly improve angiogenesis in mice with hindlimb ischemia
type: item
description: Introduction: Both ETV-2 and HGF factors are known to be important factors which trigger neo-angiogenesis both in vitro and in vivo models. This study aimed to treat hindlimb ischemia in mice by co-injection of ETV-2 and HGF viral vectors. Methods: ETV-2 and HGF vectors were prepared per previous publications. The mouse ischemic hindlimb model was performed by ligating and burning the artery at the hindlimb. ETV-2 and HGF viral vectors were co-injected into the ligated and burnt sites. Results: The results showed that co-injection of the vectors significantly improved angiogenesis as well as reduced leg loss in mice compared to placebo treatment. The percentage of mice who succumbed to ischemia was also significantly reduced compared to control. Conclusion: Overall, this study suggests a potential impact of combining ETV-2 and HGF to treat angiogenesis. Use of ETV-2 and HGF viral vectors can be a promising therapy for ischemia treatment in the future.
subject: Research Articles
  topic: section
subject: Hindlimb ischemia
  topic: keywords
subject: ETV2
  topic: keywords
subject: HGF
  topic: keywords
subject: Gene therapy
  topic: keywords
subject: Gene therapeutics
  topic: keywords
subject: Lentiviral vectors
  topic: keywords
subject: Regenerative medicine
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
subject: Editor's Pick
  topic: disciplines
creator: Phuc Van Pham
creator: Ngoc Bich Vu
creator: Thuy Thi-Thanh Dao
creator: Ha Thi-Ngan Le
creator: Lan Thi Phi
creator: Ngoc Kim Phan
rights: Copyright (c) 2017 Phuc Van Pham, Ngoc Bich Vu, Thuy Thi-Thanh Dao, Ha Thi-Ngan Le, Lan Thi Phi, Ngoc Kim Phan
date: 2017-07-23
publication date: 2017-07-23
starting page: 189
ending page: 200
doi: 10.15419/psc.v4i01.193

Transplantation of umbilical cord blood-derived mesenchymal stem cells to treat liver cirrhosis in mice: a comparison of tail and portal vein injection
type: item
description: Introduction: To date, there have been many studies indicating the positive effects of stem cells on treating liver cirrhosis. In this study, we used umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) for treatment in a mouse model of liver cirrhosis. Specifically, we determined and compared the effectiveness of two methods of MSC injection (tail vein versus portal vein). Methods: Liver cirrhosis in male Swiss mice (of age approximately 11 weeks or under) was induced by administration of carbon tetrachloride (CCl4; 1 ml/kg). One million UCB-MSCs were then transplanted into cirrhotic mice via the portal vein or tail vein. After 21 days, blood samples were collected for measurement of transaminase, bilirubin and albumin. The expression of fibrosis-associated genes, specifically procollagen – alpha 1 and integrin – beta1, were assessed using quantitative RT-PCR. The histopathology of the specimens was also evaluated using hematoxylin/eosin, Masson trichrome staining, and immunohistochemistry using collagen type 1 and alpha-SMA antibodies. Results: After 21 days, cirrhotic mice treated with UCB-MSCs showed recovery of bilirubin index, increase of liver albumin synthesis, inhibition of fibrosis-related gene expression (e.g. procollagen – alpha 1 and integrin – beta1), and remodeling of liver histology. From comparison of the different routes of transplantation, UCB-portal route was significantly more effective than UCB-tail route at reducing aspartate transaminase (AST) activity and bilirubin index (P<0.05), and inhibiting procollagen – alpha 1 and integrin – beta1 expression (P<0.05). UCB-MSCs from both transfusion routes showed accelerated improvement of liver histopathology. Conclusion: Therapeutic strategies using UCB-MSCs have proven to be promising for the treatment of liver cirrhosis. Injection of UCB-MSC via portal vein was more effective than tail vein for cirrhosis treatment.
subject: Research Articles
  topic: section
subject: Liver disease
  topic: keywords
subject: liver fibrosis
  topic: keywords
subject: mesenchymal stem cell
  topic: keywords
subject: stem cell transplantation
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
subject: Editor's Pick
  topic: disciplines
creator: Trinh Van Le
creator: Nam Hai Nguyen
creator: Huy Quang Do
creator: Huy Minh Le
creator: Nhung Hai Truong
rights: Copyright (c) 2017 Trinh Van Le, Nam Hai Nguyen, Huy Quang Do, Huy Minh Le, Nhung Hai Truong
date: 2017-09-10
publication date: 2017-09-10
starting page: 201
ending page: 216
doi: 10.15419/psc.v4i2.365

Concise review: Hematopoietic stem cell transplantation to treat insulin-dependent diabetes mellitus
type: item
description: Diabetes mellitus is a lifelong disease which causes negative effects on patient life. As a result of the disease, patients suffer from high blood sugar and the inability to obtain energy effectively from food. Diabetes mellitus can lead to life-threatening complications such as blindness, cardiovascular disease and diabetic coma. Diabetes can arise from defective insulin hormone production (usually related to type 1 diabetes mellitus or insulin-dependent diabetes mellitus (IDDM)) and/or defective insulin hormone function (related to type 2 diabetes mellitus). IDDM usually occurs in young people and can cause many serious health problems. There are several treatments which been investigated and applied in patients. Among them, stem cell therapy (especially hematopoietic stem cell therapy) has great potential to treat diabetes due to its many distinct advantages. The review will focus on diabetes mellitus, hematopoietic stem cells, and the novel hematopoietic stem cell therapy in diabetes mellitus type 1 treatment.
subject: Reviews
  topic: section
subject: Hematopoietic stem cell transplantation
  topic: keywords
subject: Hematopoietic stem cells
  topic: keywords
subject: Insulin-dependent diabetes mellitus
  topic: keywords
subject: stem cell
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Anh Thi-Van Bui
creator: Phuc Van Pham
rights: Copyright (c) 2017 Anh Thi-Van Bui, Phuc Van Pham
date: 2017-11-28
publication date: 2017-11-28
starting page: 217
ending page: 226
doi: 10.15419/psc.v4i2.381

Conversion of human adipose derived stem cells into endothelial progenitor cells
type: item
description: Introduction: Endothelial cells (ECs) or endothelial progenitor cells (EPCs) are essential cells for blood vascular regeneration and vascular tissue engineering. However, the source of EPCs are limited. Indeed, these cells only existence with low rate at some tissues such as bone marrow, umbilical cord blood and peripheral blood. This study aimed to produce EPCs from direct reprogramming of adipose tissue-derived mesenchymal stem cells (ADSCs) by ETV2 transfection in vitro. Methods: ADSCs were isolated according to the published works. They were confirmed as mesenchymal stem cells (MSCs) with some characteristics included expression of CD44, CD73, CD90, negative of CD14, CD45, and HLA-DR; in vitro differentiation into adipocytes, and osteoblasts. ETV-2 mRNA was in vitro produced by commercial kit. ETV-2 mRNA molecules were transfected into ADSCs by Fugenes and Lipofectamine agents. These transfected cells were evaluated the expression of EPC properties included expression of CD31, VEGFR-2 in the cell surface by flow cytometry, immunocytochemistry, and in vitro vessel formation in the Matrigel. Results: The results showed that ETV-2 could transform the ADSCs from mesenchymal cell phenotype into endothelial cell phenotype with 10% transfected ADSCs expressing the CD31 in their surface, they also could form the vessel structure in vitro. Conclusion: Although the low efficacy of direct reprogramming, this study gave the new strategy to produce EPCs from the favorite cell sources as ADSCs.
subject: Research Articles
  topic: section
subject: stem cell
  topic: subjects
subject: regenerative medicine
  topic: subjects
creator: Van Hong Tran
creator: Hoa Trong Nguyen
creator: Phuc Van Pham
rights: Copyright (c) 2017 Van Hong Tran, Hoa Trong Nguyen, Phuc Van Pham
date: 2017-11-29
publication date: 2017-11-29
starting page: 229
ending page: 240
doi: 10.15419/psc.v4i3.396

Concise review: Umbilical cord derived mesenchymal stem cell bank
type: item
description: Mesenchymal stem cells (MSCs) are the most promising stem cells for clinical applications. MSCs are widely used in disease treatment in some different countries. Recently, some MSC banks are developed to cryopreserve MSCs from umbilical cord tissues, adipose tissues, and bone marrow. This review aims to discuss some techniques, some advantages, as well as disadvantages of the umbilical cord, derived mesenchymal stem cell banking (UCMSCB). From 2010 to date, there are more than 10 UCMSCBs established in the world. There are two methods to isolate UCMSCs including tissue culture and single cell culture. Then they are cryopreserved in the liquid nitrogen for a long time. Although UCMSCBs can provide more the choice to store the MSCs from the umbilical cord, allogeneic MSC transplantation with high efficacy in disease treatment suggests that UCMSCBs should change with new approaches to use the cryopreserved samples.
subject: Reviews
  topic: section
subject: Stem cell
  topic: keywords
subject: Regenerative medicine
  topic: keywords
subject: Mesenchymal stem cell bank
  topic: keywords
creator: Saharah Nievaleve
rights: Copyright (c) 2017 Saharah Nievaleve
date: 2017-12-29
publication date: 2017-12-29
starting page: 228
ending page: 233
doi: 10.15419/psc.v4i3-4.397

Expanded mesenchymal stem cell transplantation is safe in both local injection and vein transfusion
type: item
description: More than 500 clinical trials are using mesenchymal stem cells (MSCs) in the world to treat some different diseases. The safety of expanded MSC transplantation is the most important thing to ensure that this therapy can become the routine treatment of human illnesses. More than five MSCs based stem cell drug products are approved in various countries demonstrated that expanded MSCs are safe in both local injection and transfusion. Moreover, some recent reports for six years followed-up clinical trials using expanded MSCs confirmed that there is not different tumorigenesis between the patients with and without expanded MSC transplantation. This letter aims to provide some evidence about the safety of expanded MSCs in clinical applications. However, the MSC quality should be strictly controlled during the in vitro MSC expansion.
subject: Letter to Editor
  topic: section
subject: regenerative medicine
  topic: subjects
creator: Vlassov V Salval
creator: Yone Moto
rights: Copyright (c) 2017 Vlassov V Salval, Yone Moto
date: 2017-12-29
publication date: 2017-12-29
starting page: 227
ending page: 228
doi: 10.15419/psc.v4i3-4.398

An evolution of stem cell research and therapy in Viet Nam
type: item
description: Stem cell research is among the most exciting, attractive, and rapidly growing fields in biomedicine. Besides the popularity of stem cell research at the bench, stem cell therapy has become a bustling interest in the clinic and industry. In Vietnam, biomedical scientists have studied stem cells since 1995. From that time on, Vietnamese scientists have obtained significant achievements in stem cell research and therapy, especially with regards to stem cell therapy for disease treatment. This report aims to provide an overview of stem cell research and therapy from 1995 to date. Stem cell research activities were collected by questionnaire and analyzed based on publications and projects about stem cells in various databases, including Pubmed, Web of Science, Google Scholar, Embase, as well as from national scientific information. The results showed that stem cell research and therapy significantly increased from 2009 to date with greater publications on stem cells and clinical applications. With this growth rate, Vietnam is poised to continue developing in the stem cell industry. The potential goal for Vietnam is to advance as one of the countries in the Southeast Asian region and in the world that continues to push the frontier in stem cell research and therapy.
subject: Reviews
  topic: section
subject: regenerative medicine
  topic: subjects
subject: stem cell
  topic: subjects
creator: Phuc Van Pham
creator: Ngoc Bich Vu
creator: Oanh Thuy Huynh
creator: Mai Thi-Hoang Truong
creator: Truc Le-Buu Pham
creator: Long Thanh Dang
creator: Ngoc Kim Phan
creator: Kiet Dinh Truong
rights: Copyright (c) 2018 Phuc Van Pham, Ngoc Bich Vu, Oanh Thuy Huynh, Mai Thi-Hoang Truong, Truc Le-Buu Pham, Long Thanh Dang, Ngoc Kim Phan
date: 2018-01-30
publication date: 2018-01-30
starting page: 235
ending page: 250
doi: 10.15419/psc.v5i1.400

Expression of CD34 marker in the adipose tissue derived stem cells
type: item
description: Introduction: Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marker of hematopoietic stem cells. This study aimed to analyze and determine the expression of CD34 marker in ten samples of ADSCs obtained from 10 donors. Methods: All ADSC samples were isolated and expanded according to the published previous protocols. They were confirmed as the MSCs with some markers and differentiation potential, excepting the CD34 expression. Then they were cultured and analyzed the expression of CD34 by flow cytometry at passage 3, 5, 7 and 9. Results: The results showed that expression of CD34 in ADSCs was different between donors and their passages that accounted from 1.21% to 23.38%. Conclusion: These results suggested that ADSCs are not ‘truly” MSCs like MSCs from bone marrow.
subject: Research Articles
  topic: section
subject: stem cell
  topic: subjects
subject: regenerative medicine
  topic: subjects
creator: Phuc Van Pham
creator: Ngoc Bich Vu
creator: Van Hong Tran
rights: Copyright (c) 2018 Phuc Van Pham, Ngoc Bich Vu, Van Hong Tran
date: 2018-03-01
publication date: 2018-03-01
doi: 10.15419/psc.v5i1.399

Mesenchymal stem cells transplantation reduces diabetic nephropathy
type: item
description: Diabetes mellitus is a metabolic disease in which the pancreas is unable to produce enough insulin due to the destruction of b -cells or the body does not utilize insulin properly. Continuous fluctuation of blood glucose levels is responsible for prolonged complications such as diabetic nephropathy (DN), diabetic retinopathy, or diabetic cardiomyopathy. Approximately, 20-30% of all diabetic patients face DN, which causes the formation of diabetic glomerular lesions and reduced glomerular filtration rate. In the case of renal failure, kidney transplantation is the only available therapy, however, it is expensive and almost unattainable due to unavailability of donors and host immune rejection. Stem cells are an alternative and attractive source of therapy because of their proliferative nature and the ability to produce distinct specialized cells. Mesenchymal stem cells (MSCs), which are derived from bone marrow, possess an anti-inflammatory property and the ability of selfrenewal and differentiation into a variety of specialized cells. MSCs are widely used to treat different diseases including DN and they have shown encouraging outcomes. This review provides details about the regenerative efficiency of using MSCs in treating diabetic nephropathy.
subject: Reviews
  topic: section
subject: Diabetes mellitus
  topic: keywords
subject: Diabetic Nephropathy
  topic: keywords
subject: Stem cells
  topic: keywords
subject: Regeneration
  topic: keywords
subject: Mesenchymal stem cells
  topic: keywords
subject: Biotechnology and Biochemistry
  topic: disciplines
creator: Abeer Kazmi
creator: Arsheema Kazmi
creator: Wali Muhammad
creator: Muhammad Azhar
rights: Copyright (c) 2019 Abeer Kazmi, Arsheema Kazmi, Wali Muhammad, Muhammad Azhar
date: 2019-03-31
publication date: 2019-03-31
starting page: 260
ending page: 268
doi: 10.15419/psc.v6i1.403

Therapeutic role of bone marrow-derived stem cells and zinc sulfate for reduction of liver fibrosis
type: item
description: Introduction: Hepatic fibrosis is considered as the initial state of any chronic hepatic disease; progression causes accumulation of extracellular matrix (ECM) and develops into cirrhosis. In case of liver failure, transplantation was considered as the only suitable therapy but due to the hurdles in transplantation, therapy using stem cells was promoted. Mesenchymal stem cells (MSCs) are widely used for the treatment of a variety of diseases. Oxidative stress at the damage site causes poor MSC proliferation and engraftment. Thus, it is necessary to induce antioxidants with MSCs to enhance potency. Methods: To explore the therapeutic potential of zinc sulfate (ZnSO4) and MSCs on carbon tetra chloride (CCl4)-induced hepatic toxicity, 6-8 week-old female albino mice (BALB/c) were used. Initially, mice were infected with CCl4 and then intra-peritoneally injected with MSCs only, ZnSO4 only, or a combination of MSCs and ZnSO4. MSCs were isolated from femur and tibia of mice, and were cultured under control conditions. Results: The morphological results revealed that in contrast to MSC-only therapy, ZnSO4 enhanced the therapeutic potential of MSCs when administered to CCl4-injured mice. Biochemically, level of serum Alanine Transaminase (ALT) and total bilirubin was found to be significantly decreased in ZnSO4 + MSC transplanted mice. Histopathological examination also revealed that ZnSO4 + MSC transplantation induced a strong anti-apoptotic effect on CCl4-injured liver. Reverse transcription polymerase chain reaction (RT-PCR) detected a noteworthy anti-fibrotic effect of MSCs in the presence of ZnSO4, down-regulation of apoptotic marker (Bax), and up-regulation of anti-apoptotic (Bcl-xl) and hepatic (Albumin) markers. Conclusion: Thus, it was concluded that the presence of ZnSO4 reduced oxidative stress, enhanced the proliferation rate of MSCs, and significantly attenuated hepatic fibrosis.
subject: Research Articles
  topic: section
subject: Albumin
  topic: keywords
subject: Bax
  topic: keywords
subject: Bcl-xl
  topic: keywords
subject: Liver Fibrosis
  topic: keywords
subject: Mesenchymal stem cells
  topic: keywords
subject: Zinc sulfate
  topic: keywords
subject: b -Actin
  topic: keywords
subject: stem cell
  topic: subjects
subject: regenerative medicine
  topic: subjects
creator: Arsheema Kazmi
creator: Abeer Kazmi
creator: Sulaiman Shams
creator: Abdul Sajid
creator: Khalid Khan
rights: Copyright (c) 2019 Arsheema Kazmi, Abeer Kazmi, Sulaiman Shams, Abdul Sajid, Khalid Khan
date: 2019-12-21
publication date: 2019-12-21
starting page: 269
ending page: 278
doi: 10.15419/psc.v6i1.406

https://cellstemcell.org/index.php/PSC/article/view/407
type: item
title: Isolation of cancer stem-like cells from hepatocellular carcinoma cell line HepG2 by methods of magnetic-activated cell sorting, spheroid culture, and anti-tumor drug-resistant selection: A primary evaluation
description: Introduction: Recently reported data have suggested that only a small subset of cancer cells possess the capability to initiate malignancies. These observations were based on investigation of cells within the primary tumors displaying a distinct surface marker pattern. CD133 marker is a putative hematopoietic and neuronal stem cell marker, which is also considered to be a tumorigenic marker in brain, prostate and liver. Recent studies have shown that a small population of CD133-positive cells, indeed, exists in human hepatocellular carcinoma (HCC) cell lines and primary HCC tissues. This study was aimed at isolating the cancer stem-like cells from hepatocellular carcinoma cell line HepG2 using three different methods: magnetic-activated cell sorting (MACS), spheroid culture (SC), and anti-tumor drug (ATD) resistant selection. Methods: HepG2 hepatocellular carcinoma cells were expanded to yield enough cells that could be used to isolate cancer stem-like cells by these three methods. For MACS, cancer stem-like cells were sorted using anti-CD133 monoclonal antibody. For the second method, cancer stem-like cells were enriched by selection of anti-tumor drug resistance property. Lastly, for the third method, three-dimensional (3D) culture was used to enrich for the cancer stem-like cells. The cells obtained by the three methods were expanded to obtain an adequate number of cells for confirmation of CD133 expression. Results: The expression of CD133+ cells in the three methods was found to be different. In the MACS method, the expanded CD133+ sorted cells cultured through 2 passages only contained 0.40 % CD133+ cells. In the 3D spheroid cell culture, of the population of cells there were 38.39 % that were CD133+ cells. Lastly, in the anti-tumor drug (doxorubicin at 150 nM) resistant selection, 66.22 % were CD133+ cells. Conclusion: This study shows that isolation of HepG2 derived CD133+ population by culture with doxorubicin (150 nM) yields the highest efficiency and puri…
subject: Research Articles
  topic: section
subject: cancer stem cell
  topic: keywords
subject: CD133
  topic: keywords
subject: doxorubicin
  topic: keywords
subject: spheroid
  topic: keywords
subject: magnetic
  topic: keywords
creator: Sinh Truong Nguyen
creator: Luong Sy Nguyen
creator: Thao Hoang Phuong Nguyen
creator: Phuc Hong Vo
creator: Nghia Minh Do
creator: Kiet Dinh Truong
creator: Phuc Van Pham
rights: Copyright (c) 2020 Sinh Truong Nguyen, Luong Sy Nguyen, Thao Hoang Phuong Nguyen, Phuc Hong Vo, Nghia Minh Do, Kiet Dinh Truong, Phuc Van Pham
date: 2020-03-18
publication date: 2020-03-18
starting page: 279
ending page: 289
doi: 10.15419/psc.v7i1.407

Ovarian cancer cells with CD133+ phenotype is more resistant against Ngai Bun Boesenbergia pandurata extract than original ovarian cancer cells
type: item
description: Introduction: Ovarian cancer is one of the most common cancers in women. Due to the difficulty in early detection and treatment of ovarian cancer, many research studies and clinical trials have been developed to discover more efficient therapies. Besides Western medicine, traditional medicine has gained increased interest as a research field with potential to lead to the production of marketable therapeutic products. With the diversity of tropical plants in Asia, traditional medicine has been very popular and has served as a traditional therapy for generations. The Ngai bun (Boesenbergia pandurata) root is used not only as a food spice but also in ethnomedicine. This study aimed to compare the anti-tumor activity of Boesenbergia pandurata root extract against ovarian cancer cells and CD133+ ovarian cancer cells that were enriched from the original ovarian cancer cells. Methods: Crude extract of Boesenbergia pandurata roots were prepared in two kinds of solvents (methanol and chloroform). The ovarian cancer cells OVP-10 were used in this study. The population of CD133+ ovarian cancer cells (CD133+OVP-10) were sorted from the OVP-10 cancer cells. Both OVP-10 cells and CD133+OVP-10 cells were treated with these crude extracts. Adiposederived stem cells (ADSCs) were used as control normal cells for all assays. The anti-tumor activity of extracts were evaluated based on the IC50 values. Results: Based on the IC50 index, the chloroform extract had an anti-tumor activity higher than that of methanol extract, on both OVP-10 and CD133+OPV-10 cells (IC50 of methanol and chloroform extracts were 330.1 +/- 16.9 ug/mL and 246.5 +/- 21.2 ug/mL, respectively, for OVP-10 cells; IC50 of methanol and chloroform extracts were 411.8 +/- 83.7 ug/mL and 307 +/- 9.2 ug/mL respectively, for CD133+OVP-10 cells). The results also showed that CD133+OVP-10 cells were more resistant to chloroform extract than were OVP-10 cells (307 +/- 9.2 mg/mL vs. 246.5 +/- 21.2 mg/mL, respectively, for CD133…
subject: Research Articles
  topic: section
subject: CD133 cancer stem cells
  topic: keywords
subject: ovarian cancer stem cells
  topic: keywords
subject: ovarian cancer
  topic: keywords
subject: Boesenbergia pandurata
  topic: keywords
subject: biology
  topic: subjects
subject: biomedicine
  topic: subjects
creator: Oanh Thi-Kieu Nguyen
creator: Phuc Van Pham
rights: Copyright (c) 2020 Oanh Thi-Kieu Nguyen, Phuc Van Pham
date: 2020-03-19
publication date: 2020-03-19
starting page: 290
ending page: 295
doi: 10.15419/psc.v7i1.408

Induced pluripotent stem cells are Japanese brand sources for therapeutic cells to pretrial clinical research
type: item
description: iPSCs are promising and have potential benefits for medical use, understanding human organogenesis, and cell therapy for advanced diseases. iPSCs are derived pluripotent cells which can further differentiate into functional human cell-lineages, such as neuronal, epithelial cells, cardiac cell, immune cell, and blood cells, etc. Thirteen years on, the discovery of iPSC has revolutionized the field of regenerative medicine, and also the number of clinical studies using iPSC has been growing rapidly worldwide. However, Japan is leading the race of iPSC-based studies and clinical trials due to government support. The Japanese government implemented the world’s fastest approval system and set to host first pretrial clinical studies using iPSC derived therapeutic products. Also, multinational companies of Japan are investing enormously in iPSC-based research for mobilization of iPSC-derived regenerative products to the research institution globally. This review presents an overview of iPSCs, potential benefits, commercialization of iPSC, iPSC-based pretrial clinical studies, and iPSC biobanking in Japan.
subject: Reviews
  topic: section
subject: : Induced pluripotent stem cells
  topic: keywords
subject: Therapeutic cells
  topic: keywords
subject: Clinical trials
  topic: keywords
subject: Biobank
  topic: keywords
subject: Japan
  topic: keywords
creator: Rupendra Shrestha
rights: Copyright (c) 2020 Rupendra Shrestha
date: 2020-06-08
publication date: 2020-06-08
starting page: 296
ending page: 303
doi: 10.15419/psc.v7i1.409

Using Defined Neural Cell Populations as a Possible Solution for Challenges in Neural Stem Cell Therapy
type: item
description: Neural stem cells exist in the mammalian nervous system. Despite extensive research to improve methods for isolation, propagation and differentiation of these cells, the clinical application of the in vitro expanded neural stem cells has remained challenging. These challenges include heterogeneity of neural stem cell progeny, limited neuronal cell yield both in number and phenotype, paucity of oligodendroglial cells, and predominant astroglial differentiation in vitro and after transplantation. Moreover, uncontrolled proliferation and tumorigenicity of the undifferentiated progeny possibly limit the clinical application of neural stem cells. Here, we propose using defined neural cell populations as a main solution.
subject: Commentaries
  topic: section
subject: Stem cell
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
creator: Hassan Azari
rights: Copyright (c) 2014 Progress in Stem Cell
date: 2014-09-20
publication date: 2014-09-20
starting page: 03
ending page: 06

Recent Progress in Stem Cell Chondrogenesis
type: item
description: Significant efforts have been undertaken in the last decade in the development of stem cell-based therapies for cartilage repair. To improve the clinical efficacy of stem cells for articular cartilage repair, understanding the factors and conditions that influence stem cell chondrogenesis in their lineage-specific differentiation and phenotypic stability of the cartilage formation following differentiation would be necessary. In this progress update, we discuss the use of the various sources of stem cells, induction factors including growth factors, oxygen tension and biomaterial scaffolds, and some of the cell-cell/matrix interactions and underlying mechanisms that regulate stem cell chondrogenesis towards stable cartilage formation.
subject: Reviews
  topic: section
subject: Stem cell
  topic: keywords
subject: Regenerative medicine
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
subject: Editor's Pick
  topic: disciplines
creator: Wei Seong Toh
rights: Copyright (c) 2014 Wei Seong Toh
date: 2014-09-21
publication date: 2014-09-21
starting page: 07
ending page: 17

Demons resurrected from Angels! What are or are not the molecular clues of the rising of the teratoma or teratocarcinoma from human amniotic stem cell?
type: item
description: Stem cell has great therapeutic potentials as it proliferates indefinitely, as well as gives rise to other cell type in our body. However, human pluripotent stem cell (hPSC) technology faces some obstacles associated with tumorigenicity and telomere shortening. And the risk of tumorigenicity of hPSC upon transplantation is one of the major hurdles, which must be overcome before hPSC based clinical practices. Interestingly, human amniotic stem cell (hASC) showed promising results by bypassing from the drawback. But, the important question is how hASC fully or partially escape from the progression of teratoma in severe combined immunodeficiency (SCID) mice, which remains unravel. It is decisive to comprehend the molecular mechanisms responsible for that of teratogenic and this non-teratogenic effect. Evidently, teratoma represents a critical line between stem cells, differentiation and tumorigenicis. And hASC typifies as a transitional stage between human embryonic stem cell (hESC) and adult stem cell. Hence, the study of hASC as a comparative model to reverse teratoma/teratocarcinoma formation and stem cell pluripotency to deciphering the molecular signals pathways promoting teratoma by hESC/hiPSC but not by hASC. Overall, learning the mechanisms of teratoma formation reversely by inducing in hASC could provide the knowledge that improves the tissue reconstitution potential from hESC and human induced PSC.
subject: Research Articles
  topic: section
subject: stem cell
  topic: keywords
subject: cancer
  topic: keywords
subject: Cancer
  topic: subjects
creator: Md. Shaifur Rahman
creator: Madhuri Haque
creator: S. M. Asaduzzaman
rights: Copyright (c) 2015 Md. Shaifur Rahman, Madhuri Haque, S. M. Asaduzzaman
date: 2015-12-25
publication date: 2015-12-25
starting page: 58
ending page: 64

Adipose tissue derived stromal vascular fraction transplantation can recover spinal cord injury in mice
type: item
description: Introduction: Stem cell therapy is one of the most promising therapies for degenerative diseases and related injuries. Adipose tissue derived stem cells (ADSCs) exhibit some particular properties such as high production of paracrine factors. Indeed, ADSCs have been successfully used to treat diseases, including osteoarthritis, diabetic ulcer, etc. Methods: In this study, ADSCs were used to treat spinal cord injury (SCI) in a mouse model. Non-expanded ADSCs, from stromal vascular fractions (SVFs) isolated from both autologous and allogeneic adipose tissues, were injected into injured sites of mice at a specified dose. The SCI mouse model were generated by transection of spinal cord at vertebrae T8 - T10. After 1 week of transection, mice exhibiting completed SCI were divided into 4 groups: group 1 was control (mice without any treatment), group 2 was placebo (mice treated with platelet rich plasma (PRP)), group 3 was allogeneic SVF transplantation (mice treated with allogeneic SVFs), and group 4 was autologous SVF transplantation (mice treated with autologous SVFs). For the treatment groups, mice were transplanted with 20 µL of activated PRP or/and with 106 cells of SVF (allogeneic or autologous) into the injured position through laminectomy. The recovery of SCI was evaluated by locomotor test, sensory test and sensory-motor test at 5 weeks after transplantation. The histology of the spinal cord also was checked after 5 weeks. Results: The results showed that in all groups with PRP injected with or without SVFs, the inflammation was efficiently controlled. The glial scar as well as myelin defragmentation were clearly reduced. However, a significant improvement of BBB score was only recorded in mice transplanted with autologous SVFs. Conclusion: The results of our study show that autologous SVF transplantation in combination with PRP can be a promising therapy for SCI.
subject: Research Articles
  topic: section
subject: Adipose derived stem cells
  topic: keywords
subject: Mesenchymal stem cells
  topic: keywords
subject: Spinal cord injury
  topic: keywords
subject: Stromal vascular fraction
  topic: keywords
subject: stem cell
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
subject: Editor's Pick
  topic: disciplines
creator: Hau Thi-My Lam
creator: Minh Nguyen-Thu Tran
creator: Khoa Anh Bui
creator: Thao Thi-Thu Le
creator: Khanh Hong-Thien Bui
creator: Ngoc Kim Phan
creator: Phuc Van Pham
rights: Copyright (c) 2016 Hau Thi-My Lam, Minh Nguyen-Thu Tran, Khoa Anh Bui, Thao Thi-Thu Le, Khanh Hong-Thien Bui, Ngoc Kim Phan, Phuc Van Pham
date: 2016-12-21
publication date: 2016-12-21
starting page: 144
ending page: 158
doi: 10.15419/psc.v3i04.148

Autologous and allogeneic transplantation of adipose derived stem cells have similar efficacy for type 1 diabetes mellitus therapy in mouse models
type: item
description: Introduction: Type 1 diabetes mellitus (T1D) disease is caused by lesions or dysfunction of beta cells of pancreatic islets, causing less insulin to be secreted into the blood and thereby increasing glucose levels in the blood. In this study, we evaluated and compared the efficiency of treatment for T1D using autograft and allograft adipose-derived stem cells (ADSCs). Methods: ADSCs were collected from the belly of mice before they were injected using a single dose of streptozotocin (100 mg/kg) to induce T1D. T1D mice were intravenously injected with a dose of 2x106 ADSCs into the tail vein. Therapeutic efficacy was assessed by survival rate, blood glucose levels, serum insulin levels, histology and immunohistochemistry of pancreatic islets. Results: The results showed that both autograft and allograft transplantation of ADSCs demonstrated similarities in mortality rate, blood glucose level, blood insulin level, quantity and size of pancreatic islets. Both transplantations significantly improved T1D mice, which showed a decrease in mortality rate as well as blood glucose level, and increases in blood insulin level, quantity and size of pancreatic islets. Conclusion: The similar results suggest that both autologous and allogeneic transplantations of ADSCs are promising therapy for T1D treatment.
subject: Research Articles
  topic: section
subject: stem cell
  topic: keywords
subject: regenerative medicine
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
subject: Editor's Pick
  topic: disciplines
creator: Anh Nguyen-Tu Bui
creator: Oanh Thi-Kieu Nguyen
creator: Cong Le-Thanh Nguyen
creator: Loan Thi-Tung Dang
creator: Dung Phuong Nguyen
creator: Phuc Van Pham
creator: Ngoc Kim Phan
rights: Copyright (c) 2016 Anh Nguyen-Tu Bui, Oanh Thi-Kieu Nguyen, Cong Le-Thanh Nguyen, Loan Thi-Tung Dang, Dung Phuong Nguyen, Phuc Van Pham, Ngoc Kim Phan
date: 2016-12-20
publication date: 2016-12-20
starting page: 129
ending page: 143
doi: 10.15419/psc.v3i04.142

Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector
type: item
description: Introduction: Embryonic stem cells are pluripotent, thus capable of differentiating into all types of cells derived from the three germ layers. However, the application of embryonic stem cells (ESCs) for preclinical and clinical studies is difficult due to ethical concerns. Induced pluripotent stem cells (iPSCs) are derived from differentiation and have many ESC characteristics. The study herein examines the production of iPSCs from reprogramming of mouse embryonic fibroblasts (MEFs) via transduction with defined factors. Methods: MEFs were collected from mouse embryos via a previously published protocol. The cells were transduced with a single polycistronic viral vector encoding mouse cDNAs of Oct3/4, Sox2, Klf4 and c-Myc. Transduced cells were treated and sub-cultured with ESC medium. The cells were evaluated as iPSCs with specific morphology, and expression SSEA-1, Oct3/4, Sox2 and Nanog. In addition, they also were evaluated for pluripotency by assessing alkaline phosphatase (AP) activity and in vivo teratoma formation. Results: Under the reprogrammed conditions, the transduced cells displayed a change in morphology, forming ESC-like clusters. These cell clusters strongly expressed pluripotent markers as well as ESC-specific genes. Furthermore, the colonies exhibited higher AP activity and formed teratomas when injected into the murine testis. Conclusions: The study herein suggests that MEFs can be reprogrammed into iPSCs using a polycistronic viral vector encoding mouse cDNAs for Oct3/4, Sox2, Klf4 and c-Myc.
subject: Methodologies
  topic: section
subject: Polycistronic vectors
  topic: keywords
subject: Epigenetic reprogramming
  topic: keywords
subject: iPSCs
  topic: keywords
subject: Fibroblast reprogramming
  topic: keywords
subject: regenerative medicine
  topic: keywords
subject: Regenerative Medicine
  topic: subjects
subject: Editor's Pick
  topic: disciplines
creator: Oanh Thuy Huynh
creator: Mai Thi-Hoang Truong
creator: Phuc Van Pham
rights: Copyright (c) 2017
date: 2017-06-12
publication date: 2017-06-12
starting page: 159
ending page: 174
doi: 10.15419/psc.v4i01.176

Dissecting asthma pathogenesis through study of patterns of cellular traffic indicative of molecular switches operative in inflammation
type: item
description: Background: Inflammation and degeneration are the two edged swords that impale a pulmonary system with the maladies like asthma and idiopathic pulmonary fibrosis. To explore critical role players that orchestrate the etiology and pathogenesis of these diseases, we used various lung disease models in mice in specific genetic knockout templates. Materials and methods: Acute and chronic allergic asthma and idiopathic pulmonary fibrosis model in mouse was developed in various genetic knockout templates namely α4Δ/ Δ (α41-/-), β2-/-, and α4-/- β2 mice, and the following parameters were measured to assess development of composite asthma phenotype- (i) airway hyperresponsiveness to methacholine by measuring lung resistance and compliance by invasive and Penh by non-invasive plethysmography as well as lung resistance and compliance using invasive plethysmography, (ii) in situ inflammation status in lung parenchyma and lung interstitium and also resultant airway remodeling measured by histochemical staining namely Masson’s Trichrome staining and Hematoxylin & Eosin staining, (iii) formation of metaplastic goblet cells around lung airways by Alcian blue dye, (iv) measurement of Th1 and Th2 cytokines in serum and bronchoalveolar lavage fluid (BALf), (v) serum allergen-specific IgE. Specifically, ovalbumin-induced acute allergic asthma model in mice was generated in WT (wildtype) and KO (knockout) models and readouts of the composite asthma phenotype viz. airway hypersensitivity, serum OVA-specific IgE and IgG, Th2 cytokine in bronchoalveolar lavage fluid (BALf) and lymphocyte cell subsets viz. T, B cells, monocytes, macrophages, basophils, mast cells and eosinophils (by FACS and morphometry in H&E stained cell smears) were assessed in addition to lung and lymph node histology. Results: We noticed a pattern of cellular traffic between bone marrow (BM)à peripheral blood (PB) à lung parenchyma (LP) à (BALf) in terms of cellular recruitment of key cell sub-types critical for onset…
subject: Research Articles
  topic: section
subject: stem cell
  topic: keywords
subject: biology
  topic: keywords
subject: cancer
  topic: keywords
creator: Ena Ray Banerjee
rights: Copyright (c) 2015 Ena Ray Banerjee
date: 2015-12-25
publication date: 2015-12-25
starting page: 28
ending page: 57